Gene cloning
Learning objectives
- To illustrate the different steps required to clone a gene of interest.
- To understand how a gene can be transferred to a host cell.
- To understand how the host cells having the recombinant DNA are selected.
Cloning a gene is multi-step process involving the isolation of the gene of interest from one given organism, then the transfer of the gene of interest to a host cell which is capable of producing large quantities of recombinant protein.
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