Indirect enzyme-linked immunosorbent assay (ELISA)
Learning objectives
- To understand the principle behind the use of the ELISA technique in screening for AIDS.
- To describe the different steps invloved in the ELISA technique.
- To point out the differences between direct ELISA and indirect ELISA.
ELISA, Enzyme Linked Immunosorbent Assay, is a is an immuno-enzymatic technique that enables the detection of antigen-antibody reactions thanks to a reaction with colored products. ELISA is a technique currently used in the process of screening for HIV infections.
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